The Metabolism of Acetaminophen and the Synthesis of Glutathione Download Scientific Diagram Tylenol's Impact on Glutathione and Liver Damage Not only that, it depletes your #glutathione levels! Tylenol's Impact on Glutathione and Liver Damage Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen Induced Hepatotoxicity: a Comprehensive Update