Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: Figure 1 from Acetaminophen toxicity and 5 oxoproline (pyroglutamic acid): a tale of two cycles, one an ATP depleting futile cycle and the other a useful cycle. Semantic Scholar Glutathione, Tylenol & Your Health: What You Should Know Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Reactive Metabolite induced Protein Glutathionylation: A Potentially Novel Mechanism Underlying Acetaminophen Hepatotoxicity* Molecular & Cellular Proteomics